In this way, we incorporated 83 996 super-enhancers that had been experimentally discovered or computationally identified in 134 cell types, tissues, or diseases from humans, mice, D. melanogaster and C. elegans (Table 1). In the case of humans, there were 75 439 super-enhancers from 99 cell types/tissues including 30 disease cell lines/tissues. The current release of SEA incorporates 83 996 super-enhancers computationally or experimentally identified in 134 cell types/tissues/diseases, including human (75 439, three of which were experimentally identified), mouse (5879, five of which were experimentally identified), Drosophila melanogaster (1774) and Caenorhabditis elegans (904). To facilitate data extraction, SEA supports multiple search options, including species, genome location, gene name, cell type/tissue and super-enhancer name. At present, there are several methods for discovering super-enhancers, including computational method based on factors such as Med1 (19), BRD4 (10) and H3K27ac (1), and experimental technology such as ChIA-PET (8), CRISPR/Cas9 (9), RT-qPCR (23) and so on.

Besides, we manually collected eight super-enhancers that had been identified and confirmed by experimental methods in published papers. 19) to identify the super-enhancers in a broad range of samples from multiple species. Because of the larger size of super-enhancers, it was difficult and time consuming to experimentally identify novel super-enhancers from a range of cell types/tissues/diseases (12). It is known that H3K27ac can identify a large fraction of super-enhancers formed by the master transcription factors and it has been profiled in a broad range of samples from multiple species (4). Thus, H3K27ac can be used as the surrogate mark of super-enhancers and imported into ROSE software developed by Loven et al. Wedding is an event that happens once in your life time and it is the time when you tie a knot with your dream partner. When a successful music composer is called for an event, it is undeniable for them to learn more about what kind of an event it actually is and who are the people who would be attending this event.

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The response provides detailed (epi)genetic information, incorporating cell type specificity, nearby genes, transcriptional factor binding sites, CRISPR/Cas9 target sites, evolutionary conservation, SNPs, H3K27ac, DNA methylation, gene expression and TF ChIP-seq data. Super-enhancers are genome regions that are large clusters of transcriptional enhancers (1). The term ‘super-enhancer’ was used for the first time by Chen et al. Moreover, analytical tools and a genome browser were developed for users to explore super-enhancers and their roles in defining cell identity and disease processes in depth. Users can input multiple options to the query engine to acquire the super-enhancers. It enable users to do genomic region enrichment analysis and cell type specificity analysis. All search and analysis results can be downloaded as flat format for further analysis. What actions can you take to create a more accessible interface? Step 1: First of all, make sure you are owning the item, Boombox and can access it freely without any problems or issues. With the first child, parents face new challenges – and need answers to completely new questions.